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Subnuclear localization of the SUVR proteins

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posted on 30.12.2011, 16:43 by Tage Thorstensen, Andreas Fischer, Silje V. Sandvik, Sylvia S. Johnsen, Paul E. Grini, Gunter Reuter, Reidunn B. Aalen

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Taken from "The SUVR4 protein is a nucleolar histone methyltransferase with preference for monomethylated H3K9"

Nucleic Acids Research 2006;34(19):5461-5470.

Published online 04 Oct 2006

PMCID:PMC1636477.

© 2006 The Author(s)

Root cells from seedlings expressing the indicated SUVR-GFP fusion proteins (green) were investigated using fluorescence microscopy. Nuclei were stained with DAPI (blue) to visualize heterochromatin (left panels). The nucleolus is indicated by an arrow, and spots at the edge of the nucleolus with an arrowhead. () SUVR1a-GFP. The insert in the right panel shows, as a control, the nucleolus of a cell expressing SUVR1a-GFP (green) exposed to FISH with an 18S rDNA probe (red). () SUVR1b-GFP. () SUVR2a-GFP. () SUVR4a-GFP localization pattern found in the majority of cells inspected. () Alternative subnuclear localization of SUVR4a-GFP. () GFP fused to the N-terminus (40 amino acid) of SUVR1a. () GFP fused to the C-terminus (27 amino acid) of SUVR4. () GFP fused to SUVR4 without the C-terminus.

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