Subcellular localization and disruption of PML bodies by wild-type and mutant IE1.

(A) TetR (w/o), TetR-IE2 and TetR-IE1 cells expressing the indicated HA-tagged wild-type (wt) or mutant IE1 proteins were treated with dox for 24 h. Indirect immunofluorescence staining was performed using mouse anti-HA and rabbit anti-PML combined with goat anti-mouse Alexa Fluor 488 and goat anti-rabbit Alexa Fluor 594 antibodies. DAPI was used to stain DNA. Representative merge images from cells showing the localization of IE1, IE2 and PML relative to DNA are presented (Keyence BZ-9000 microscope, 40× objective). The percentage of cells exhibiting predominantly disrupted or intact PML bodies is shown in Fig 3C.

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