pone.0204709.g002.tif (462.38 kB)
Download file

Study design to examine the anti-inflammatory properties of GM-1111 in a murine model of CRS.

Download (462.38 kB)
posted on 25.09.2018, 17:47 by Jeremiah A. Alt, Won Yong Lee, Brock M. Davis, Justin R. Savage, Thomas P. Kennedy, Glenn D. Prestwich, Abigail Pulsipher

At week 0, control animals (PBS group) were sensitized with an intraperitoneal (i.p.) injection of PBS vehicle in alum adjuvant, whereas the A. fumigatus + PBS (red arrow) and A. fumigatus + GM-1111 (blue arrow) groups received A. fumigatus extracts in alum adjuvant. After 1 week, the animals were subjected to an 8-week regiment (3 times weekly) of intranasal PBS or A. fumigatus extracts. At week 5, intranasal treatment of GM-1111 or PBS was initiated (5 times weekly) and continued for 4 weeks with continued A. fumigatus extract administration (upper right inset). At week 9, whole blood was collected, and the animals were sacrificed and examined for histologic changes and inflammatory tissue biomarkers associated with CRS.