Structural alterations of VLN flaps after IR injury.
A-G HE-stained (A, D and F) and immunohistochemical (B, C, E and G) sections of control (A-C), IR-45 (D and E) and IR-120 (F and G) VLN flaps. SS = subcapsular sinus, IS = intermediate sinus, MS = medullary sinus, F = follicle. Arrowhead = LYVE-1+ cell-lined sinus. C (insert of B) Asterisk = main efferent lymphatic vessel. H-J HE-stained section of follicles of control (H), IR-45 (I) and IR-120 (J) lymph nodes. K Lymph node cellularity (nuclei mm-2). Mean ± SEM, n = 7–8, *P < 0.05, **P < 0.001. L Adipose tissue cellularity (nuclei mm-2). Mean ± SEM, n = 7–8, n.s. = not significant. Scale bars: A, B, D-G = 300 μm, C = 40 μm, H-J = 60 μm. HE = hematoxylin and eosin, LYVE = lymphatic vessel endothelial hyaluronan receptor, VLN = vascularized lymph node.