Structural alterations of VLN flaps after IR injury.
figure
posted on 2020-10-06, 17:27 authored by Florian S. Frueh, Bijan Jelvani, Claudia Scheuer, Christina Körbel, Bong-Sung Kim, Pietro Giovanoli, Nicole Lindenblatt, Yves Harder, Emmanuel Ampofo, Michael D. Menger, Matthias W. Laschke<p><b>A-G</b> HE-stained (<b>A, D</b> and <b>F</b>) and immunohistochemical (<b>B, C, E</b> and <b>G</b>) sections of control (<b>A-C</b>), IR-45 (<b>D</b> and <b>E</b>) and IR-120 (<b>F</b> and <b>G</b>) VLN flaps. SS = subcapsular sinus, IS = intermediate sinus, MS = medullary sinus, F = follicle. Arrowhead = LYVE-1<sup>+</sup> cell-lined sinus. <b>C</b> (insert of <b>B</b>) Asterisk = main efferent lymphatic vessel. <b>H-J</b> HE-stained section of follicles of control (<b>H</b>), IR-45 (<b>I</b>) and IR-120 (<b>J</b>) lymph nodes. <b>K</b> Lymph node cellularity (nuclei mm<sup>-2</sup>). Mean ± SEM, n = 7–8, *<i>P</i> < 0.05, **<i>P</i> < 0.001. <b>L</b> Adipose tissue cellularity (nuclei mm<sup>-2</sup>). Mean ± SEM, n = 7–8, n.s. = not significant. Scale bars: <b>A, B, D-G</b> = 300 μm, <b>C</b> = 40 μm, <b>H-J</b> = 60 μm. HE = hematoxylin and eosin, LYVE = lymphatic vessel endothelial hyaluronan receptor, VLN = vascularized lymph node.</p>
