Streptavidin pulldown of biotinylated proteins in mESCs and mESC-derived neurons.
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posted on 2025-03-04, 18:22 authored by Lisa Feicht, Aaron Dangel, Ralf-Peter JansenMicroID fusion protein (70 kDa, arrow) was detected by an anti-GFP antibody in total cell lysates (left lanes) and after enrichment by pulldown (right lanes).
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randomly integrated microidmouse hipp11 locusmhipp11 locus resultsidentifying proteins associatedtransgenic biotin ligasesmodified biotin ligasebiotin ligasetransient transfectionstem cellsspecific organellepromoter usedpowerful methodderived neuronscorrect localizationbiotinylation activitybait protein10 days
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