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Stem peptide specificity of SpOatAC and SaOatAC.

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posted on 27.10.2017, 17:38 by David Sychantha, Carys S. Jones, Dustin J. Little, Patrick J. Moynihan, Howard Robinson, Nicola F. Galley, David I. Roper, Christopher G. Dowson, P. Lynne Howell, Anthony J. Clarke

A. Stacked and offset ESI-mass spectra of mutanolysin-treated products from reactions of 10 μg·mL-1 of (left to right) muroglyan-5P, muroglycan-4P, and muroglycan-3P in 50 mM sodium phosphate buffer pH 6.5 incubated with 0.5 mM pNP-Ac in the absence (control) and presence of the respective enzyme (10 μM). The major O-acetylated products are labeled in blue which are 42.01 m/z units larger than the respective unmodified PG monomer. B. MS/MS analysis of the major product ions identified in the respective panels above and C, interpretation of the corresponding fragment ions.