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SteC is sufficient to cause ROCK-like F-actin reorganization

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posted on 2011-12-30, 20:01 authored by John Poh, Charlotte Odendall, Ad Spanos, Cliona Boyle, Mei Liu, Paul Freemont, David W Holden

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Taken from "SteC is a kinase required for SPI-2-dependent F-actin remodelling"

Cellular Microbiology 2008;10(1):20-30.

Published online Jan 2008


© 2007 The Authors Journal compilation © 2007 Blackwell Publishing Ltd

A. Representative confocal images of Swiss 3T3 cells transfected with vectors expressing myc-tagged SteC, C-SteC, SteCK256H or active ROCK (ROCK-K). Cells were immunolabelled with anti-Myc antibody (green in merged image) and F-actin was visualized by phalloidin-RRX staining (red in merged image). Bars represent 10 μm. B. Quantification of transfected cells displaying F-actin reorganization (white bars) and also periphery F-actin reorganization (black bars). Results are the means ± SE of three independent experiments, in which a total of 300 transfected cells were examined for each transfection vector.


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