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Stage-specific PbSERA-promoter-driven protein expression.

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posted on 2020-09-21, 17:43 authored by Elyzana Dewi Putrianti, Anja Schmidt-Christensen, Volker Heussler, Kai Matuschewski, Alyssa Ingmundson

The indicated 5’ regions upstream of the PbSERA4 or PbSERA5 coding sequences were cloned in front of GFP into the P. berghei transfection plasmid pL0031 (right). GFP expression in P. berghei ANKA parasites transfected with these constructs was monitored across the life cycle stages: midgut-oocysts (oo), salivary gland sporozoites (sp), HepG2 cells infected with transgenic exo-erythrocytic parasites at indicated timepoints (EE) and erythrocytic stage (ES) were stained with Hoechst 33342 and visualized by live microscopy.