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Download fileStage-specific PbSERA-promoter-driven protein expression.
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posted on 2020-09-21, 17:43 authored by Elyzana Dewi Putrianti, Anja Schmidt-Christensen, Volker Heussler, Kai Matuschewski, Alyssa IngmundsonThe indicated 5’ regions upstream of the PbSERA4 or PbSERA5 coding sequences were cloned in front of GFP into the P. berghei transfection plasmid pL0031 (right). GFP expression in P. berghei ANKA parasites transfected with these constructs was monitored across the life cycle stages: midgut-oocysts (oo), salivary gland sporozoites (sp), HepG2 cells infected with transgenic exo-erythrocytic parasites at indicated timepoints (EE) and erythrocytic stage (ES) were stained with Hoechst 33342 and visualized by live microscopy.
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generation Pb SERA 4-specific antiseraparasite-encoded cysteine proteaseblood cell infectionhosts bridges malaria transmissionliver-stage merozoite releasePb SERA 4-deficient parasitesPlasmodium berghei serineblood stagesPlasmodium parasitesPbSERA 4 resultsblood stagePlasmodium life cycleblood-stage parasitesPlasmodium cysteine proteasePb SERA 4blood-stage infectionliver-stage developmentwildtype Pasexual blood-stage replicationTargeted disruptionliver infection stageantigen 4papain-like cysteine protease familyCysteine protease activitydefect resultsliver-stage Plasmodium egress
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