pone.0239325.g002.tif (1.49 MB)

Stabilization of induced neural stem cells with tet-On transactivator gene.

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posted on 18.09.2020, 17:30 by Kamila Rosiak-Stec, Dagmara Grot, Piotr Rieske

Representative images of (A) undifferentiated iNSc tet expressing neural precursor markers SOX2 (green) and nestin (red), and (B) differentiated iNSc tet expressing astroglia marker GFAP (green) and neuronal-specific marker Map2 (red). Cell nuclei were counterstained with the nuclear DNA marker DAPI (blue). Scale bars represent 50 μm (A-B). (C) Induction of green fluorescent protein (GFP, green) expression in iNSc tet-GFP treated with doxycycline (1 μg/mL, Dox) for 96 hours. Cell nuclei were counterstained with the nuclear DNA marker DAPI (blue). Scale bars represent 100 μm. (D, E) Quantification of GFP fluorescence intensity in iNSc tet-GFP after 48 and 72 hours of incubation with doxycycline at different concentrations. Statistical significance calculated by One-way ANOVA with Dunnett’s multiple comparison test. Error bars indicate SEM. **, p<0.01; ***, p<0.005; ns, not significant.

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