Stability of mtDNA inheritance.
Clone #3 was re-cloned in either selective medium devoid of uridine and pyruvate (-UP) or in non-selective medium containing uridine and pyruvate (+UP) after 3 weeks of growth in non-selective medium. Subclones grown in either selective (A) or non-selective (B) medium were tested for the presence of mtDNA by PCR. C. mtDNA copy number was determined in 5 clones grown in either selective (-UP) or non-selective (+UP) conditions, and means were compared (D). ns, not significant, two-tailed Student’s t-test assuming unequal variance. E. mtDNA copy number was determined in six selected clones after 20-day propagation in either selective (-UP) or non-selective (+UP) media.