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Src-FAK signaling blockade suppressed IL-6/sIL-6R-induced tube formation of SV-LECs.

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posted on 06.07.2016 by Yu-Han Huang, Hung-Yu Yang, Shiu-Wen Huang, George Ou, Ya-Fen Hsu, Ming-Jen Hsu

(A) SV-LECs were seeded on Matrigel in the presence of VEGF-C (50 ng/ml) or IL-6 plus sIL6R (20 ng/ml) with or without PP2. Cells were then photographed under phase-contrast after 3 h. A representative microscopic phenotype of the formed tubes is shown (N = 3). (B) Total tube length of the formed capillary-like tube under different treatments as described in (A) was also measured using pen type digital meter. Each column represents the mean ± S.E.M. of three independent experiments. (C) SV-LECs were seeded on Matrigel in the presence of IL-6 plus sIL6R (20 ng/ml) with or without NSC 667249. Cells were then photographed under phase-contrast after 3 h. Total tube length of the formed capillary-like tube under different treatments was measured using pen type digital meter. Each column represents the mean ± S.E.M. of three independent experiments. * p<0.05, compared to the control group; # p<0.05, compared to the vehicle-treated group in the presence of IL-6 plus sIL6R.

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