Validation of specificy of HHIP antibody. 16HBE cells were seeded in duplicate, transfected with or without HHIP overexpression plasmid and harvested at 24, 48 and 72 hours after the transfection. Only upon overexpressin of HHIP protein, the protein band of HHIP is observed, starting at 24h and disappearing after 72h after overexpression.