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Specifically in the setting of C. rodentium infection, Irgm1 is required to prevent apoptosis of C-LP monocytes and transitioning monocytes.

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posted on 2020-05-26, 17:40 authored by Gregory A. Taylor, Hsin-I Huang, Brian E. Fee, Nourhan Youssef, Mark L. Jewell, Viviana Cantillana, Alexi A. Schoenborn, Allison R. Rogala, Anne F. Buckley, Carl G. Feng, Bruce A. Vallance, Ajay S. Gulati, Gianna E. Hammer

(A,B) Co-housed, littermate Irgm1+/+ and Irgm1-/- mice were infected with C. rodentium and analyzed 10 days later for Annexin V and 7AAD expression on C-LP monocytes. Representative flow plot of C-LP monocytes from mice of the indicated genotype (A) and the percentage of Annexin V+ or Annexin V+7AAD+ monocytes from all mice tested (B). (C,D) C-LP monocytes from day 6 of DSS treated mice were analyzed as above. (E,F) C. rodentium infected mice from (A) were analyzed for Annexin V and 7AAD expression on C-LP transitioning monocytes. Representative flow plot of C-LP transitioning monocytes from mice of the indicated genotype (E) and the percentage of Annexin V+ or Annexin V+7AAD+ transitioning monocytes from all mice tested (f). (G,H) C-LP transitioning monocytes from day 6 of DSS treated mice were analyzed as in (E,F). Each dot represents one mouse. Error bars represent mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, n.s., not significant (unpaired Student’s t test). Data are representative of three independent experiments each including at least 5 mice of each genotype.

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