Specifically in the setting of C. rodentium infection, Irgm1 is required to prevent apoptosis of C-LP monocytes and transitioning monocytes.

(A,B) Co-housed, littermate Irgm1^+/+ and Irgm1^-/- mice were infected with C. rodentium and analyzed 10 days later for Annexin V and 7AAD expression on C-LP monocytes. Representative flow plot of C-LP monocytes from mice of the indicated genotype (A) and the percentage of Annexin V⁺ or Annexin V⁺7AAD⁺ monocytes from all mice tested (B). (C,D) C-LP monocytes from day 6 of DSS treated mice were analyzed as above. (E,F) C. rodentium infected mice from (A) were analyzed for Annexin V and 7AAD expression on C-LP transitioning monocytes. Representative flow plot of C-LP transitioning monocytes from mice of the indicated genotype (E) and the percentage of Annexin V⁺ or Annexin V⁺7AAD⁺ transitioning monocytes from all mice tested (f). (G,H) C-LP transitioning monocytes from day 6 of DSS treated mice were analyzed as in (E,F). Each dot represents one mouse. Error bars represent mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, n.s., not significant (unpaired Student’s t test). Data are representative of three independent experiments each including at least 5 mice of each genotype.