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Specific 3H-tiotidine binding to cultured neonatal rat astrocytes.

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posted on 09.05.2016, 05:58 by Mojca Kržan, Robert Vianello, Aleksandra Maršavelski, Matej Repič, Maja Zakšek, Kristina Kotnik, Estera Fijan, Janez Mavri

3H-tiotidine binds to a single population of binding sites with a KD of = 4.7 ± 1.0 nM and Bmax of 18.6 ± 1.7 fmol/mg protein. The deuterated medium does not significantly change the binding parameters of 3H-tiotidine to astrocytic H2 receptor binding sites—KD changed to 7.4 ± 2.4 nM and Bmax dropped to 17.1 ± 2.7 fmol/mg protein.

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