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Spastin depletion impairs respiration and growth in zebrafish.

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posted on 21.04.2020 by Yoan Arribat, Dogan Grepper, Sylviane Lagarrigue, Timothy Qi, Sarah Cohen, Francesca Amati

(A) Representation of Spastin deletion in CRISPR/Cas9 knockout zebrafish. (B) Spastin protein truncation resulting from CRISPR/Cas9 in KO model. (C) Dechorionation efficiency rate in 60 hpf embryos wild-type (Ctrl) compared with Spastin KO (Spa-/-). N = 3 experiments; n > 200 embryos per group. (D-E) Impact of Spastin deletion on oxygen consummation rate (OCR) and extra-cellular acidification rate (ECAR) in 48 hpf embryos. n = 11 embryos per group. (F-H) Length, weight, and Body Mass Index in 3 months old adult zebrafish. n = 7 animals per group. Bars are mean ± SEM, #P = 0.07, *P < 0.05, **P < 0.01, ***P<0.001 (unpaired t‐test).