pgen.1008665.g002.tif (1.2 MB)

Spastin depletion impairs respiration and growth in zebrafish.

Download (1.2 MB)
figure
posted on 21.04.2020 by Yoan Arribat, Dogan Grepper, Sylviane Lagarrigue, Timothy Qi, Sarah Cohen, Francesca Amati

(A) Representation of Spastin deletion in CRISPR/Cas9 knockout zebrafish. (B) Spastin protein truncation resulting from CRISPR/Cas9 in KO model. (C) Dechorionation efficiency rate in 60 hpf embryos wild-type (Ctrl) compared with Spastin KO (Spa-/-). N = 3 experiments; n > 200 embryos per group. (D-E) Impact of Spastin deletion on oxygen consummation rate (OCR) and extra-cellular acidification rate (ECAR) in 48 hpf embryos. n = 11 embryos per group. (F-H) Length, weight, and Body Mass Index in 3 months old adult zebrafish. n = 7 animals per group. Bars are mean ± SEM, #P = 0.07, *P < 0.05, **P < 0.01, ***P<0.001 (unpaired t‐test).

History

Licence

Exports