Spastin depletion impairs respiration and growth in zebrafish.
figure
posted on 2020-04-21, 22:05 authored by Yoan Arribat, Dogan Grepper, Sylviane Lagarrigue, Timothy Qi, Sarah Cohen, Francesca Amati<p>(A) Representation of Spastin deletion in CRISPR/Cas9 knockout zebrafish. (B) Spastin protein truncation resulting from CRISPR/Cas9 in KO model. (C) Dechorionation efficiency rate in 60 hpf embryos wild-type (Ctrl) compared with Spastin KO (Spa-/-). N = 3 experiments; n > 200 embryos per group. (D-E) Impact of Spastin deletion on oxygen consummation rate (OCR) and extra-cellular acidification rate (ECAR) in 48 hpf embryos. n = 11 embryos per group. (F-H) Length, weight, and Body Mass Index in 3 months old adult zebrafish. n = 7 animals per group. Bars are mean ± SEM, <sup>#</sup><i>P =</i> 0.07, *<i>P</i> < 0.05, **<i>P</i> < 0.01, ***P<0.001 (unpaired <i>t</i>‐test).</p>
History
Related Materials
- 1. DOI - Is supplement to Spastin mutations impair coordination between lipid droplet dispersion and reticulum
Usage metrics
Keywords
Spastin depletionresults showHSPSpastin M 1Spastin mutationsnovel biomarkersorganelle componentssystem resultsintracellular dispersionlipid droplet dispersionorganelle dynamicsHereditary Spastic ParaplegiaMTcoordinates LD dispersionSpastin modulates transcripts levelsm 1 isoformssplice variantsendoplasmic reticulumreticulum Lipid dropletsREEP 1.ER shapeimpacts lipids profileimpact LD fatesubcellular locationSpastin depletion influencesLD dispersionimpacts LD network
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC