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Snail- and Slug-induced EMT promoted drug resistance of parental PC9 and HCC827 cells.

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posted on 06.07.2017, 17:38 by An-Fu Lee, Man-Chin Chen, Chao-Ju Chen, Chih-Jen Yang, Ming-Shyang Huang, Yu-Peng Liu

(A) The protein levels of EMT biomarkers and ectopically expressed Slug, Snail and Twist1 in the parental PC9 cells. (B) The pLex-, pLex-Slug- and pLex-Snail-expressing parental PC9 cells were treated with gefitinib at the indicated concentrations for 72 hours, and the relative cell viability was determined by MTT assay. (C) The protein levels of EMT biomarkers and the ectopically expressed Slug, Snail and Twist1 in the parental HCC827 cells. (D) The pLex-, pLex-Slug- and pLex-Snail-expressing parental HCC827 cells were treated with gefitinib at the indicated concentrations for 72 hours, and the relative cell viability was determined by MTT assay. (E) Endogenous E-cadherin (CDH1) was knocked down by two shRNA clones (#1 and #2). The protein levels of EMT biomarkers were examined by Western blot. (F) Stable clones of scramble shRNA-, shRNA #1- and shRNA #2-transduced PC9 cells were treated with gefitinib at the indicated concentrations for 72 hours, and the relative cell viability was determined by MTT assay.

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