Small compounds attenuate the virulence of H. pylori via inhibiting urease activity.
(A) Viability of gastric cells exposed to H. pylori. (B) Images of AGS cells after exposure (24 h) to H. pylori with or without supplementation of AHA, CBS, cmpd4, and cmpd8. CBS and cmpd4/cmpd8-treated H. pylori cells show less toxicity to gastric cells. (C) Amounts of ammonia in cell culture medium after exposure of gastric cell to H. pylori. Urea was supplied to allow a final concentration of 10 mM. Reduced levels of ammonia are noted in CBS- and cmpd4/cmpd8-treated groups. The underlying data for Fig 5A and 5C can be found in S1 Data. AHA, acetohydroxamic acid; CBS, colloidal bismuth subcitrate.