Sleep phenotype of female flies with mutant dbt and lower expression of dbt.

(A) Sleep profiles of dbt^S female flies. n (CS) = 45, n (dbt^S) = 39. (B) Average sleep amount during the daytime and nighttime in dbt^S female flies. (daytime: t = 5.556, df = 82, ***p <0.0001 by unpaired t-test; nighttime: t = 9.431, df = 82, ***p <0.0001 by unpaired t-test). n (CS) = 45, n (dbt^S) = 39. (C) Sleep profiles of dbt^L female flies. n (CS) = 48, n (dbt^L) = 44. (D) Average sleep amount during the daytime and nighttime in dbt^L female flies (daytime: t = 5.945, df = 90, ***p<0.0001 by unpaired t-test; nighttime: t = 3.426, df = 90, ***p = 0.0009 by unpaired t-test). n (CS) = 48, n (dbt^L) = 44. (E) Sleep profile of female flies with down-regulated dbt expression from tim-GAL4. n (UAS-dbt^RNAi/+) = 42, n (UAS-dicer/+; tim-GAL4/+) = 47, n (UAS-dicer/+; tim-GAL4/+; UAS-dbt^RNAi/+) = 47. (F) Average sleep amount during daytime and nighttime with down-regulated dbt expression from tim-GAL4 driver. n (UAS-dbt^RNAi/+) = 42, n (UAS-dicer/+; tim-GAL4/+) = 47, n (UAS-dicer/+; tim-GAL4/+; UAS-dbt^RNAi/+) = 47. (G) Sleep profile of female flies with down-regulated dbt expression from per-GAL4. n (UAS-dicer/+; UAS-dbt^RNAi/+) = 46, n (per-GAL4/+) = 45, n (UAS-dicer/ per-GAL4; UAS-dbt^RNAi/+) = 45. (H) Average sleep amount during daytime and nighttime with down-regulated dbt expression from per-GAL4 driver. n (UAS-dicer/+; UAS-dbt^RNAi/+) = 46, n (per-GAL4/+) = 45, n (UAS-dicer/ per-GAL4; UAS-dbt^RNAi/+) = 45. (I) Loss of expression of dbt in α, β and γ lobes of mushroom body (201Y) of flies cause a sleep increase during the daytime. n (UAS-dicer/+; UAS-dbt^RNAi/+) = 47, n (201Y/+) = 48, n (UAS-dicer/201Y; UAS-dbt^RNAi/+) = 38. (J) Average sleep amount during the daytime and nighttime with down-regulation of dbt expression with mushroom body driver 201Y. n (UAS-dicer/+; UAS-dbt^RNAi/+) = 47, n (201Y/+) = 48, n (UAS-dicer/201Y; UAS-dbt^RNAi/+) = 38. (K) Loss of expression of dbt in α and β lobe of mushroom body (C309) of flies causes a sleep increase during the daytime. n (UAS-dicer/+; UAS-dbt^RNAi/+) = 42, n (C309/+) = 41, n (UAS-dicer/C309; UAS-dbt^RNAi/+) = 44. (L) Average sleep amount during the daytime and nighttime with down-regulation of dbt expression with mushroom body driver C309. n (UAS-dicer/+; UAS-dbt^RNAi/+) = 42, n (C309/+) = 41, n (UAS-dicer/C309; UAS-dbt^RNAi/+) = 44. (M) Loss of expression of dbt in fan-shaped body (C205) leads to a sleep increase during the daytime. n (UAS-dicer/+; UAS-dbt^RNAi/+) = 47, n (C205/+) = 46, n (UAS-dicer/+; UAS-dbt^RNAi/C205) = 44. (N) Average sleep amount during the daytime and nighttime with down-regulated expression of dbt with fan-shaped body driver C205. n (UAS-dicer/+; UAS-dbt^RNAi/+) = 47, n (C205/+) = 46, n (UAS-dicer/+; UAS-dbt^RNAi/C205) = 44. (O) Loss of expression of dbt in ellipsoid body (C819) leads to a sleep increase during the daytime. n (UAS-dicer/+; UAS-dbt^RNAi/+) = 41, n (C819/+) = 48, n (UAS-dicer/+; UAS-dbt^RNAi/C819) = 42. (P) Average sleep amount during the daytime and nighttime with down-regulated expression of dbt with ellipsoid body driver C819. n (UAS-dicer/+; UAS-dbt^RNAi/+) = 41, n (C819/+) = 48, n (UAS-dicer/+; UAS-dbt^RNAi/C819) = 42. ns. no significant difference, *p< 0.05, **p< 0.01 and ***p< 0.001. Black and purple bars (or lines) respectively represent data of wildtype and dbt mutant in A-D, in E-P, Black and grey bars (or lines) respectively represent UAS-/+, -GAL4/+ control flies, while purple bars (or lines) represent the treatment flies. The horizontal bar below each graph presents day (white) and night (black).

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