Single-nucleus transcriptomic analysis on the hippocampus of <i>Cyfip2</i>^{<i>+/R87C</i>} mice.

<p><b>(A)</b> Violin plots showing quality control metrics for each sample: the total number of Unique Molecular Identifier (UMI) counts (left), the number of observed genes (middle), and percent of mitochondrial genes (right). KI, knock-in. <b>(B)</b> UMAP visualization of all hippocampal cells, grouped by sample. Each sample is represented by a distinct color. <b>(C)</b> Volcano plot showing global differential expression (DE) analysis results for all hippocampal cells based on genotype. <b>(D)</b> Subclustering results of neurons. The left panel presents a UMAP visualization of neuronal subtypes, while the right panel shows a dot plot of cell-specific marker gene expression, with dot size representing the percentage of cells expressing the gene and color intensity indicating average expression levels. <b>(E)</b> Subclustering results of excitatory neurons. The left panel displays a UMAP visualization of excitatory neuronal subtypes, while the right panel features a dot plot highlighting cell-specific marker genes. <b>(F)</b> Subclustering results of pyramidal neurons. The left panel presents a UMAP visualization of neuronal subtypes, while the right panel shows a dot plot of cell-specific marker gene expression. <b>(G)</b> Bar plot showing significant GSEA results for CA1-1 compared to CA1-0. The <i>Y</i>-axis represents Gene Ontology Biological Process (GOBP) terms, while the <i>X</i>-axis represents Normalized Enrichment Scores (NES). Color intensity reflects the significance level, represented by −Log₁₀ (adjusted <i>P</i> value).</p> <p>(TIF)</p>