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Short-read and long-read RNA sequencing of mouse hematopoietic stem cells at bulk and single-cell levels

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posted on 18.08.2021, 03:57 by Xiuran Zheng

Hematopoietic stem cells (HSCs) lie at the top of the differentiation hierarchy. Although HSC and their immediate downstream, multipotent progenitors (MPP) have full multilineage differentiation capacity, only long-term (LT-) HSC have the capacity for long term self-renewal. The heterogeneity within the HSC population is more and more acknowledged with single-cell RNA sequencing and lineage tracing technologies. Transcriptional and post-transcriptional regulations play important roles in controlling the differentiation and self-renewal capacity within HSC population. Here we report two datasets comprising single-cell and bulk short- and long-read RNA sequencing for long- and short-term HSC and MPP isolated from mouse bone marrows. These datasets provide a groundwork for comprehensive and comparative studies on gene expression and unique alternative splicing events occur in these 3 populations while exploring the heterogeneity within all three populations.

Funding

the National Key Research and Development Program of China, Stem Cell and Translational Research

the National Science Fund for Excellent Young Scholars

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