Figure S1 - Serotonin Receptors Expressed in Drosophila Mushroom Bodies Differentially Modulate Larval Locomotion

Expression of RNAi for 5HT_1B, 5HT₂ and 5HT₇ receptors in MB γ-lobe neurons using a different Gal4 driver line cause an increased in motor output. The different RNAi were expressed under the control of MB247-Gal4, a driver line that only labels γ-lobe neurons in flies at the larval stage (Pauls et al, 2010). Results show that only 5HT_1B, 5HT₂ and 5HT₇ receptors increase locomotion, while no effect is observed in animals expressing the RNAi for 5HT_1A in MB γ-lobe. These data further confirm our hypothesis that this MB neuronal subpopulation is responsible for the effects of this manipulation on locomotion. Data shown represents mean+SEM of at least 9 different animals. *, **, ***, indicate p<0.05 compared to respective controls.

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