Sequence specific visual detection of LAMP reactions by addition of cationic polymers-2
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posted on 2011-12-31, 05:49 authored by Yasuyoshi Mori, Tsuyoshi Hirano, Tsugunori NotomiCopyright information:
Taken from "Sequence specific visual detection of LAMP reactions by addition of cationic polymers"
BMC Biotechnology 2006;6():3-3.
Published online 10 Jan 2006
PMCID:PMC1373654.
Copyright © 2006 Mori et al; licensee BioMed Central Ltd.
NA recognition probes by DNA-PEI complex (Mw of PEI is 600). When 0.2 μmol to 1.0 μmol of PEI was added as a monomer, almost 100% of labeled probes hybridized to the LAMP products for lambda DNA was taken up by the DNA-PEI complex. On the other hand, when 0.4 μmol to 0.8 μmol of PEI was added as a monomer to a reaction solution in which an amplification reaction did not take place, a small amount (