Sequence-specificity of the TFO-induced recombination

Copyright information:

Taken from "Triplex-induced recombination and repair in the pyrimidine motif"

Nucleic Acids Research 2005;33(11):3492-3502.

Published online 16 Jun 2005

PMCID:PMC1151591.

© The Author 2005. Published by Oxford University Press. All rights reserved

() Triplex-induced recombination with a pyrimidine motif target. Cells pre-transfected with the dual plasmid containing the 18 bp A–T-rich site, pSupFTC18, were transfected the next day with lipid reagent alone or with the indicated TFOs. AG30 and SCR30 are G-rich TFOs and MIX30 has a mixed sequence. Shuttle vector rescue and analysis were performed as for . () Triplex-induced recombination with a purine motif target. Cells pre-transfected with a dual plasmid containing a 30 bp G-rich site, pSupFAR, were transfected 1 day later with lipid reagent alone or with the indicated TFOs. Analysis of recombination events was performed as above. Amidate18 and G3pUmC18 bind with high affinity to the A-rich target site in the intergenic region in pSupFTC18 but not to the G-rich site in pSupFAR. AG30 binds well to the site in pSupFAR but not to that in pSupFTC18.