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Sensitivity and specificity of the assay

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posted on 30.12.2011 by Jorge M. Naciff, Brian D. Richardson, Kerry G. Oliver, M. Lynn Jump, Suzanne M. Torontali, Kenton D. Juhlin, Gregory J. Carr, Jennifer R. Paine, Jay P. Tiesman, George P. Daston

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Taken from "Design of a Microsphere-Based High-Throughput Gene Expression Assay to Determine Estrogenic Potential"

Environmental Health Perspectives 2005;113(9):1164-1171.

Published online 12 May 2005


This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original DOI.

To determine the specificity and sensitivity of the assays, a series of multiplexed hybridization reactions were performed. Four different biotinylated complementary oligonucleotides (, and ) were hybridized at different concentrations (0, 0.1, 0.316, 1, 3.16, 10, 31.6, 100 fmol) to eight different sequence-specific microsphere sets. The data demonstrate undetectable cross-hybridization. intestinal calcium-binding protein, or calbindin 3; 17α-hydroxylase cytochrome P450; cyclophylin B or peptidylprolyl isomerase B.