Seedling growth inhibition assay.

Five-day-old seedlings of the indicated genotypes were treated with 0.05uM (low) or 1.0uM (high) flg22 peptide and grown for an additional 14 d. Fresh seedlings weights were then recorded and normalized to the average untreated weight within each genotype. A. Pectin methylesterase inhibitor (PMEI) (At5g64640) knockouts versus untreated, three (pmei-1) and four (pmei-2) biological replicates of 12 seedlings per treatment. B. F-box domain-containing gene (At5g15660) knockouts versus untreated, three (f-box-1) and two (f-box-2) biological replicates of 12 seedlings each. C. Folylpolygutamate synthetase 3 (FPGS3) (At3g55630) knockout versus untreated, three biological replicates. D. TIR-X domain-containing gene (At1g47370), three biological replicates. Asterisks summarize ANOVA results across all experiments for tests of similarity of means between the mutant genotype and wild-type plants treated with the same concentration of flg22. E. FPGS1 (At5g05980), FPGS2 (At3g10160), and FPGS3 (At3g55630) versus untreated, three biological replicates. (Tukey's simultaneous test: * P < 0.05; **P < 0.005; no asterisk, P > 0.05).