Secondary characteristics of OT-I T cells from Lm-primed and PbA-primed mice, related to Fig 6.

(A) Lm-OVA and PbA-OVA memory mice were prepared as previously described. On day 44 p.i., the spleens of multiple mice per infection were pooled, enriched, and an equal number of OT-I T cells (8,500) were transferred into separate naïve mice. The following day, mice were secondary infected with VV-OVA, and 5 days later mice were sacrificed to measure the presence of OT-I T cells. (B and C) The number of OT-I T cells in the spleen (B) and blood (C) on day 5 post-infection were plotted after the transfer of an equal amount of memory OT-I T cells. (D and E) The frequency of OT-I T cells bearing a KLRG1+, KLRG1+ IL-7Rα+ (DP), or IL-7Rα+ phenotype on the indicated days after secondary infection with either Lm-OVA (blue) or PbA-OVA (orange). (F and G) Representative KLRG1 x IL-7Rα flow plots showing the phenotype of secondary effector cells on the indicated days post-infection with Lm-OVA or PbA-OVA. Data in A-C is pooled from two independent experiments with n = 5 mice per group and analyzed with a Mann-Whitney test. Data in D-F is pooled from two independent experiments with n = 3-5 mice per group and analyzed with multiple Mann-Whitney tests. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Error bars are SD.

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