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Screening of recombinase polymerase amplification combined with lateral flow test probes.
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posted on 2022-12-12, 18:24 authored by Zheng Bian, Wenbo Liu, Junhua Jin, Yanling Hao, Linshu Jiang, Yuanhong Xie, Hongxing Zhang1: ipaH probe1, 2: ipaH probe2, N1: ipaH probe1 negative control, N2: ipaH probe2 negative control. Probe 1 showed a positive result. In negative control 1, only the control line changed color, indicating it was a valid control. Probe 2 produced very obvious false positive results.
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shared virulence genelateral flow dipstick39 ° c29 × 10food sample showedcause serious foodescherichia coli minimum detection limitefficient detection platform2 lfd detection probescommon foodshigella ipah rapid detectionwidely distributedtakes lessreaction timeprimer concentrationoptimization resultsnaked eyelfd methodborne pathogensborne diseasesalso verified25 min
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