pone.0232111.g001.tif (435.13 kB)

Schematic showing the experimental design.

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posted on 24.06.2020, 17:44 by Ramesh B. Kasetti, Pinkal D. Patel, Prabhavathi Maddineni, Gulab S. Zode

Human corneoscleral segments with an intact TM rim were dissected into 4 equal quadrants and each quadrant was cultured and treated with glaucoma-inducing factors for 7 days. Each quadrant was then either fixed, or TM tissue and conditioned medium was isolated. Fixed tissues were utilized for H&E and immunohistochemical analysis while TM tissue and conditioned medium was analyzed by Western blot.