Schematic representation of the effect of macrophage depletion during DDC diet administration.
DDC diet promotes a ductular reaction (DR) budding from resident bile ducts (rBD) chaperoned by laminin rich matrix. In PBSLipo co-treated mice (from day 7 onwards), a DR type II can be observed with CK19+ cells either forming amorphous bile ducts, clusters with pseudoluminae or rosettes, sprouting into the lobule. CLOLipo co-treatment (from day 7 onwards) preserves a type I DR, comprising confined ductular proliferates in proximity to the portal vein (PV). The newly budding CK19+ cells create more differentiated, bile ductular structures with regular concentric luminae.