Schematic representation and nucleotide sequence of prothrombin variants expressed using the vectors harbouring the mutated SV40 polyadenylation signal

Copyright information:

Taken from "The relationship between the prothrombin upstream sequence element and the G20210A polymorphism: the influence of a competitive environment for mRNA 3′-end formation"

Nucleic Acids Research 2005;33(3):1010-1020.

Published online 17 Feb 2005

PMCID:PMC549410.

© The Author 2005. Published by Oxford University Press. All rights reserved

Indicated in the figure: the mutated prothrombin upstream sequence element (USEmut); AAUAAA, prothrombin (PT) polyadenylation signal; CG/CA, prothrombin polymorphic cleavage site; 50 bp prothrombin downstream genomic sequence (DGS); cryptic 3′ cleavage site; mutated SV40 polyadenylation signal (gcgcgA); SV40 cleavage site, AC. Due to the presence of adenine residues downstream of the indicated cryptic (1–3 nt) and SV40 (1–2 nt) sites, it is not possible to ascertain the exact site of cleavage by sequencing due to the presence of the poly (A) tail. For simplicity only the last non-adenine residue is indicated as the position of the cryptic and SV40 cleavage sites.