ScRNA-seq of ovaries from control and αERKO mice.
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posted on 2024-11-21, 18:50 authored by Qicai Hu, Yiqian Gui, Congcong Cao, Jun Xie, Huiru Tang(A) InDROP libraries were sequenced, demultiplexed, normalized, and analyzed using the Seurat package in ‘R’. The processed samples clustered into 14 clusters. (B) Heatmap of top 5 markers per cluster ordered by logFc values. (C) The percentage of cell counts for each cell type in the control and KO samples. (D) Heatmap of top 10 highly expressed genes per cluster ordered by logFc values. (E) ERα depletion was verified in the knockout ovaries by Western blot. (F) The protein levels of Hsd17b7, Star, and Plin4 were detected by Western blot.
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xlink "> contextknockout (&# 945cell rna sequencinggranulosa cell populationser &# 945deletion significantly altersindividual ovarian cells&# 945granulosa cellscell viewovarian cellsovarian infertilityovarian developmentwild typetranscriptional landscapereproductive systempathways centralovulation processovulation cycleoffering insightsnovel treatmentsmolecular mechanismsmay leadkey resultskey regulatorgreb1 expressiongenes linkedfindings providedistinct oocytedeletion disruptingcompare transcriptomesaffecting genes
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