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ScECM4 glutathione binding site.

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posted on 13.10.2016, 17:55 by Mathieu Schwartz, Claude Didierjean, Arnaud Hecker, Jean-Michel Girardet, Mélanie Morel-Rouhier, Eric Gelhaye, Frédérique Favier

The catalytic cysteine C46, the residues involved in polar interactions with glutathione (GS) and the tyrosines Y224, Y301 and Y346 from the triad are represented as sticks. Polar interactions (yellow dashed lines) were observed between: E173 carboxylate group and GSH N-terminal amine group; NH of S174 and carboxylate group of GSH γ-glutamyl residue; S174 hydroxyl group and carboxylate group of GSH γ-glutamyl residue; R15 guanidinium group and carbonyl group of GSH γ-glutamyl residue; W79 indole group and carbonyl group of GSH cysteine; NHs and COs of V158 and of GSH cysteine; R155 guanidinium group and carboxylate group of GSH glycinyl residue.

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