SW480-membrane associated E-cadherin can increase when negative regulators are knocked down.
Representative images from the deconvoluted screen of αE-cadherin, 72 hours post transfection of selected duplex siRNAs (25nM, duplex # indicated in brackets) for mock, ZEB1, CDK8, SNX27, EPS8L1 and ZNF518A. Scale bar; 50 μM, 20x magnification with enlarged images for E-cadherin shown below. Hoechst staining shows cell nuclei. Mock normalised Ecad score for individual siRNA (numbered 1–4) in the deconvolution screen is shown below.