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SUR-8 misexpression in circadian neurons leads to disruption of circadian rhythms.

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posted on 11.11.2019, 18:35 by Yongbo Xue, Joanna C. Chiu, Yong Zhang

a The behavioral period of Sur-8 knockdown in clock neurons.TD2 stands for tim-GAL4, UAS-dicer2; PD2 for Pdf-GAL4, UAS-dicer2; PG80 for Pdf-GAL80. RNAi-1 indicates BL29557, and RNAi-2 indicates BL60894. The values inside bars represent total flies tested. Error bars indicate SEM. *P < 0.05, ***P < 0.001, and Tukey's Multiple Comparison Test after one-way analysis of variance. b Double-plotted actograms showing average activity of Sur-8 knockdown in all circadian neurons and PDF-positive neurons. White, lights-on; grey, lights-off. c Heterozygous SUR-8 overexpression, namely one copy of UAS and GAL4, leads to slightly lengthened behavioral period. TG4, tim-GAL4; PG4, Pdf-GAL4. Error bars indicate SEM. **P < 0.01, ***P < 0.001, Tukey's Multiple Comparison Test after one-way analysis of variance. d Homozygous SUR-8 overexpression, namely, two copies of UAS and GAL4, causes severe arrhymicity. TG4-16, tim-GAL4-16. Error bars indicate SEM. **P < 0.01, ***P < 0.001, Tukey's Multiple Comparison Test after one-way analysis of variance. e Double-plotted actograms showing average activity of homozygous SUR-8 overexpression in PDF-positive neurons.

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