SPR analysis by single-cycle kinetics of PGT151 Fab binding to differentially purified CZA97.012 SOSIP.664 trimer.

SPR sensorgrams for single-cycle kinetics of PGT151 Fab binding to 2G12- or PGT151- and SEC-purified CZA97.012 SOSIP.664 trimer are shown. For comparison, SPR with multi-cycle kinetics, was also performed (S4 Fig). The sensorgrams show the cumulative binding of PGT151 Fab at increasing concentrations to immobilized trimer in RU, after background subtraction, on the y-axis vs. time (s) on the x-axis. The curve for the binding data is shown in color according to the label code; the curve for the fitted binding function is shown as a thin black line. Each association phase was 300 s; the dissociation at the end was monitored for 3600 s. The number of replicate titrations and the fitted and calculated binding parameters are given in the table underneath the sensorgram.