SOCS3 expression of lung tissues and alveolar epithelium.
(A) SOCS3 protein levels in lung tissues determined by western blot analysis. (B) SOCS3 mRNA expressions in lung tissues. (C) Representative images of SOCS3 immunofluorescence staining (FITC-labeled green; original magnification ×400) of rat lung. Nuclei were counterstained with DAPI (blue). SOCS3 expressions of alveolar epithelium are significantly decreased after IR and restored by Gas6 treatment. IR: ischemia–reperfusion. Data are expressed as the mean ± SD (n = 4 per group). ** P < 0.01 compared with the control group; *** P < 0.001 compared with the control group; ## P < 0.01 compared with the IR group; ### P < 0.001 compared with the IR group.