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SDS-PAGE gel of recombinant protein was removed Trx-tag by thrombin

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posted on 22.05.2020, 13:14 by Ton That Huu Dat, Tran Thi Hong, Nguyen Phuong Hoa, Tran Thi Kim Dung, Vu Thi Thu Huyen, Le Minh Bui, Nguyen Thi Kim Cuc, Pham Viet Cuong
(A) Western blot analysis, lane M: marker protein (Bio-Basic); lane 1: the recombinant protein containing Trx-tag tail was hybrid to antibody anti-Trx. (B) SDS-PAGE gel of recombinant protein was removed Trx-tag by thrombin, lane M: marker protein (Bio-Basic); lane 1: the recombinant protein was not removed Trx-tag; lane 2: the recombinant protein was removed Trx-tag.

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