pone.0212629.g002.tif (1.28 MB)

SDS-PAGE gel depicting FCS1 expression and purification steps.

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posted on 25.02.2019, 18:24 by Victoria Sodré, Juscemácia Nascimento Araujo, Thiago Augusto Gonçalves, Nathália Vilela, Antonio Sergio Kimus Braz, Telma Teixeira Franco, Mário de Oliveira Neto, André Ricardo de Lima Damasio, Wanius Garcia, Fabio Marcio Squina

Lane M, Molecular Marker (Color Prestained Protein Standard, Broad Range, NEB); lane 1, culture supernatant fraction immediately after IPTG addition; lane 2, cell pellet after 4 hours of IPTG-induced expression (before lysis for purification); lane 3, leftover cell pellet after lysis; lane 4, crude extract (35 μg); lane 5, affinity chromatography fraction (10 μg); lanes 6 (2 μg), 7 (12 μg), 8 (5 μg) and 9 (3 μg) size-exclusion chromatography fractions (elution volumes are shown). See in the supplementary material the metal affinity and size-exclusion column chromatograms (S2 Fig).