Results of transfection into HK-2 cells of siRNA against claudin-2, occludin or ZO-1.
A: Immunoblots using cells treated with 10, 25, 50 of 100 nM of indicated siRNA for 72 h. Each lane contained protein from a different Transwell filter support of HK-2 cells. Immunoblots were treated with anti-claudin-2 (~22 kDa), anti-occludin (~65 kDa) or anti-ZO-1 (~210 kDa). B: Immunofluorescence microscopy of HK-2 monolayers with junctional localization of claudin-2, occludin and ZO-1 depleted by siRNA transfection. Magnification, x40.