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Results of transfection into HK-2 cells of siRNA against claudin-2, occludin or ZO-1.

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posted on 18.12.2017, 18:32 by Sua Kim, Gheun-Ho Kim

A: Immunoblots using cells treated with 10, 25, 50 of 100 nM of indicated siRNA for 72 h. Each lane contained protein from a different Transwell filter support of HK-2 cells. Immunoblots were treated with anti-claudin-2 (~22 kDa), anti-occludin (~65 kDa) or anti-ZO-1 (~210 kDa). B: Immunofluorescence microscopy of HK-2 monolayers with junctional localization of claudin-2, occludin and ZO-1 depleted by siRNA transfection. Magnification, x40.