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Response of H. contortus reconstituted receptors in Xenopus oocytes.

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posted on 14.07.2016 by Thomas B. Duguet, Claude L. Charvet, Sean G. Forrester, Claudia M. Wever, Joseph A. Dent, Cedric Neveu, Robin N. Beech

Two electrode voltage clamp experiments were performed on oocytes injected with Hco-unc-63, Hco-unc-38, Hco-acr-8, and Hco-ric-3.1, Hco-unc-74, Hco-unc-50 cRNAs. Hco-unc-29.1, unc-29.3 and unc-29.4 were combined independently with the cRNA mixture. A), B) and C) Representative recording traces from single oocytes perfused with 100 μM of the following cholinergic agonists: acetylcholine (ACh), Dimethylpiperazinium (DMPP), Pyrantel (PYR), Nicotine (NIC), Bephenium (BEPH) and Levamisole (LEV). D), E) and F) Representative recording traces from single oocytes continuously perfused with 100 μM ACh. Oocytes were perfused with the following cholinergic antagonists: D-tubocurarine (dTC, 100 μM), Dihydro-β-erythroidine (DHβE, 10 μM) and Mecamylamine (MECA, 30 μM). Black horizontal bars show the time period of agonist and or antagonist application. G), H) and I) Concentration-response curves for the L-AChR1.1, L-AChR1.3 and L-AChR1.4 for ACh (black circles) and LEV (white squares). All responses are normalized to 100 μM ACh, which corresponds to the saturating dose. The ACh and LEV 50% effective concentration (EC50) values as well as Hill coefficients are indicated in Table 2. Error bars represent SD.

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