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Rescue of sphe mutant phenotype using Gal4/UAS system.

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posted on 06.12.2017, 18:41 by Jelena Patrnogic, Vincent Leclerc

(A) drosomycin expression 24 hours PI, normalized to rp49, after immune challenge with E. faecalis (spheΔ49 vs. spheΔ49; UAS-sphe p<0.0103, spheΔ104 vs. spheΔ104;UAS-sphe p<0.0229). Controls (crtl) are w1118 wild type flies. (B) Survival rate after immune challenge with E. faecalis (spheΔ49 vs. spheΔ49; UAS-sphe p<0.0001, spheΔ104 vs. spheΔ104;UAS-sphe p<0.0001). For over expression of sphe using UAS-sphe lines we used ubiquitous Act5C>Gal4.

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