Representative examples of reperfusion events in cells subjected to 5 mM Ni²⁺.

All labeling and notations are the same as in Figure, unless indicated otherwise. Note overall blunted dynamics of Fluor-4 signal during reperfusion, including the case when no apparent [Ca²⁺]_Cy overload ever occurred (B). Arrow in C indicate the time when TO-PRO3 was added to perfusate, immediately revealing that the sarcolemmal permeabilization had already occurred.