ppat.1010322.s004.tif (3.26 MB)
Reovirus infectivity is reduced in KO cells infected with virions but not ISVPs.
figureposted on 2022-03-09, 18:30 authored by Paula Ortega-Gonzalez, Gwen Taylor, Rohit K. Jangra, Raquel Tenorio, Isabel Fernandez de Castro, Bernardo A. Mainou, Robert C. Orchard, Craig B. Wilen, Pamela H. Brigleb, Jorna Sojati, Kartik Chandran, Martin Sachse, Cristina Risco, Terence S. Dermody
WT, KO, and KO+ HBMECs were adsorbed with reovirus (A) virions or (B) ISVPs at MOIs of 10,000 or 100 particles/cell, respectively, fixed at 18 h post-adsorption, and stained for reovirus antigen using IF. Representative micrographs are shown.
rna interference screensputative host factorinfectious subvirion particlesincluding ebola virushuman immunodeficiency virushepatitis c virusassembly take placereovirus outer capsidreovirus disassembly intermediatesnpc1 transport activityuncoating within endosomescells lacking npc1reovirus penetrationreovirus attachmentreovirus ).identified npc1viral transcriptionviral coretarget cellssolubilizes cholesterolsignificantly impairedreovirus infectionproteolytically removedplasma membranepick c1new functionmediated endocytosismany virusesmammalian orthoreoviruslate endosomesfindings suggestendoplasmic reticulumendocytic pathwaycholesterol traffickingcholesterol homeostasis