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Rectal mucosal IgG envelope peptide microarray responses associated with reduced risk of infection.

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posted on 03.09.2020, 17:55 by Kier Om, Dominic Paquin-Proulx, Maria Montero, Kristina Peachman, Xiaoying Shen, Lindsay Wieczorek, Zoltan Beck, Joshua A. Weiner, Dohoon Kim, Yifan Li, Thembi Mdluli, Zhanna Shubin, Christopher Bryant, Vishakha Sharma, Andrey Tokarev, Peter Dawson, Yohann White, Oliver Appelbe, Nichole R. Klatt, Sodsai Tovanabutra, Jacob D. Estes, Gary R. Matyas, Guido Ferrari, Carl R. Alving, Georgia D. Tomaras, Margaret E. Ackerman, Nelson L. Michael, Merlin L. Robb, Victoria Polonis, Morgane Rolland, Michael A. Eller, Mangala Rao, Diane L. Bolton

HIV-1 envelope-specific IgG responses were mapped by peptide microarray for rectal secretions at peak immunogenicity (month 12.5). (A) Heatmap depicts association between peak rectal epitope-specific responses and infection risk. Hazard ratios for all peptide array epitope responses that remained in the analysis set after filtering out highly correlated responses are shown, separately by sex and in all animals (*, p<0.05). (B) Epitope-specific binding responses that were protective in males with p<0.2 are shown for each animal by vaccine arm. Outlier box plots depict median, interquartile range, and +/- 1.5 the interquartile range. Female animals are depicted by circles, male by triangles. Open symbols reflect animals that remained uninfected following the ten prespecified challenges.