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Recombinant PfTRAP undergoes phosphorylation in the presence of HepG2 and HEK 293-cell lysates

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posted on 31.12.2011 by Reetesh Raj Akhouri, Ashwani Sharma, Pawan Malhotra, Amit Sharma
Recombinant PfTRAP fragments were incubated with HepG2 cell extract and HEK 293 cell lysate in the presence of P[γ ATP]. PfTRAP fragments were immunoprecipitated using anti-PfTRAP antibody and phosphorylation status was checked through autoradiography. Lanes 1, HepG2 cell lysate; 2, A domain+HepG2 cell lysate; 3, A+RII+HepG2 cell lysate; 4, ECD+HepG2 cell lysate. ECD phosphorylation was also checked using recombinant MAPK (lane 6). MAPK was used as a control (lane 5). Lanes1, HEK 293 cell lysate; 2, A+RII domain+ 293 cell lysate; 3, A+RII+RGD+ 293 cell lysate; 4, ECD+ 293 cell lysate.

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Taken from "Role of thrombospondin-related anonymous protein in host-cell interactions"

http://www.malariajournal.com/content/7/1/63

Malaria Journal 2008;7():63-63.

Published online 22 Apr 2008

PMCID:PMC2373790.

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