Receiver Operating Characteristic (ROC) curve and Decision tree.
(A) The graph is showing the ROC curve analysis of the sensitivity and specificity of each of the 12 antigens. IBAG36-IBAG37 and IBAG95 gave the bests results. (B) The decision tree established after the first data analysis is based on the number of reactive antigens. A sample is considered as negative if reactive on up to 3 antigens and positive if reactive on at least 6 antigens. When reactive on 4 or 5 antigens the sample is further analyzed: the Sum of intensities on three antigens (IBAG36-37-95) is measured and the 12 000 raw value is used as a threshold.