Real-time spread of HRECs over the electrode in response to the PFKFB3-PFK inhibitor (PFK158).

(A) The plot shows the normalized capacitance across HRECs over time, measured at an AC frequency of 64,000 Hz for both the control group and HRECs treated with PFK158 (1 μM & 10 μM). The capacitance values were normalized using the capacitance measurement taken before the addition of the inhibitor, considered as t = 0 hour. (B) The bar graph displays the normalized capacitance for the control group and HRECs treated with PFK158 (1 μM & 10 μM) at the end of the experiment (t = 120 hours). (C) The bar graph presents the areas under the normalized capacitance curve for the control group and HRECs treated with PFK158 (1 μM & 10 μM) during the time interval of t = 0–120 hours. Significant changes are indicated by p values of symbol ****<0.0001 and *<0.05; n = 3–4 biological replicates for each group. Abbreviations: Norm (normalized) and AUC (area under the curve).