Real-time spread of HRECs over the electrode in response to the PFKFB3-PFK inhibitor (PFK158).

<p><b>(A)</b> The plot shows the normalized capacitance across HRECs over time, measured at an AC frequency of 64,000 Hz for both the control group and HRECs treated with PFK158 (1 μM & 10 μM). The capacitance values were normalized using the capacitance measurement taken before the addition of the inhibitor, considered as t = 0 hour. <b>(B)</b> The bar graph displays the normalized capacitance for the control group and HRECs treated with PFK158 (1 μM & 10 μM) at the end of the experiment (t = 120 hours). <b>(C)</b> The bar graph presents the areas under the normalized capacitance curve for the control group and HRECs treated with PFK158 (1 μM & 10 μM) during the time interval of t = 0–120 hours. Significant changes are indicated by p values of symbol ****<0.0001 and *<0.05; n = 3–4 biological replicates for each group. Abbreviations: Norm (normalized) and AUC (area under the curve).</p>