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Rapid evolution of MISTRAV and its paralog MISTR1 (NDUFA4) in primate genomes.

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posted on 28.12.2020, 20:09 authored by Mahsa Sorouri, Tyron Chang, Palmy Jesudhasan, Chelsea Pinkham, Nels C. Elde, Dustin C. Hancks

Estimated dN/dS values predicted using FreeRatio analysis in PAML [28] across primate lineages for A) MISTRAV, B) MISTR1 (NDUFA4), and C) MISTRH. Rapidly evolving lineages (dN/dS > 1 or greater than or equal to 3 nonsynonymous amino acid substitutions: synonymous amino acid substitutions) are marked by red branches. D) MISTRAV E) MISTR1 (NDUFA4), and F) MISTRH amino acid positions predicted to be rapidly evolving (colored triangles) from PAML, MEME [30], and FUBAR [31] analysis. Numbering and residue are relative to the human reference sequence. Rapidly evolving sites for G) MISTRAV (red), H) MISTR1 (NDUFA4) (red), and I) MISTRH (yellow) mapped onto the predicted structure of MISTR1 (NDUFA4). Models were generated using SWISS-MODEL (https://swissmodel.expasy.org/) based on the published structure of Complex IV of the ETC containing MISTR1/NDUFA4 (PDB:5Z62) [33]. J) Model of MISTRAV (blue) within Complex IV structure (silver). ETC, electron transport chain; IMS, intermembrane space; MISTRAV, MItochondrial STress Response AntiViral; MISTRH, MItochondrial STress Response Hypoxia; NDUFA4, NADH dehydrogenase ubiquinone 1 alpha subcomplex subunit 4.

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